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All participants completed a questionnaire before commencing this study.
Local ethical approval was obtained before commencing this study and, as appropriate, tissue was collected with informed consent.
Finally, ethical approval will be sought from relevant human research ethics committees (HREC) before commencing this study.
Seven laboratories were experienced in the 2-day ELISA MN assay before commencing this study, and 6 laboratories were experienced in the 3-day HA MN assay.
Because genetic background has been reported to modify the action of this transgene (Steshina et al., 2006), we backcrossed Gtl2LacZ mice for multiple generations with 129Sv stock before commencing this study.
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We commenced this study before we had the results of one of our recent multicentre studies on pulse pressure variation/cardiac index/MAP guided intraoperative management [ 35].
We did attempt to minimize this by allowing at least a month to practice before commencing the study and permitting advice to be sought from project leaders throughout the study period.
Each participating centre obtained approval from its local ethics review committee, before commencing the study.
Before commencing the study, thorough discussions on the issues affecting the watermills' existence in the region were made with the remotely residing watermill owners.
Animals recovered for 14 days before commencing the study protocol.
A review protocol was not published before commencing the study.
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