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All animals were introduced to hydrated diet (prepared as described above) 24 36 h before commencing the experiment.
The surface soil in the plot area in Fedis site before commencing the experiment is a silty clay loam, containing 36% sand, 45% silt, and 19% clay.
In general, after reaching the desired temperature the larvae were kept undisturbed for the next 36 h before commencing the experiment and after this a batch of 30 larvae was introduced into each respective respiratory chamber.
Chambers containing 1 refuge and 1 tadpole from the appropriate treatment (Bd+ or Bd−, and predator cue were left overnight to acclimate before commencing the experiment.
The developmental stage of several representative flasks of worms was confirmed microscopically before commencing the experiment.
Animals were acclimatized to the experimental environment for 7 days before commencing the experiment (22 ± 5°C with humidity control and dark and light cycle of 12 h).
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The initial tension was adjusted to 1.5 g and an equilibration period of 90 min was allowed before commencing the experiments.
Before commencing the experiments, the participants were instructed to stop looking at the rubber hand and start looking at the knife as soon as it was presented to make sure that they perceived the threat.
Ten minutes before commencing the experiments, each bee was checked for intact PER by touching one antenna with a toothpick that had been dipped into 1M sucrose solution without allowing feeding.
The animals were allowed to acclimatize for 5 days before commencing the experiments.
The apparatus and viewing parameters were the same as in Experiment 1. Observers performed a training block, as in Experiment 1. Error rates were checked to be below 5% before commencing the main experiment.
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before resuming the experiment
before initiating the experiment
before commencing the experimental
before commencing the course
before commencing the survey
before commencing the interview
before commencing the measurement
before commencing the literature
before commencing the study
before commencing the dose response
before commencing the training
before commencing the analysis
before commencing the process
before commencing the provision
before commencing the intervention
before commencing the program
before commencing the counting
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