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Cardiomyocytes were initially stimulated to contract at 0.5 Hz for 5 min to ensure a steady-state before commencing the frequency response.
All records (T1) were taken in the centric relation (CR) before commencing the intervention.
Each participating centre obtained approval from its local ethics review committee, before commencing the study.
All antagonists were introduced 20 min before commencing the dose response curves.
Animals recovered for 14 days before commencing the study protocol.
A review protocol was not published before commencing the study.
Multicentre Research Ethics Committee MRECC) approval was obtained before commencing the study.
The animal then remained on the platform for 30 s before commencing the next trial.
Before commencing the study, permission was obtained from the University of Manitoba Research Ethics Committee and from the recruitment sites.
In addition, the improved vision would enhance the clinicians' confidence in diagnosis before commencing the treatment.
An informed verbal consent was taken before commencing the interview and reviewing the blood test report.
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