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Nematostella embryos (collected at various time points), Hydra adults (including animals that were undergoing the process of budding) and Trichoplax from laboratory cultures (animals were starved for 24 h before collection) were collected, frozen in liquid nitrogen, and stored at -80°C.
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The type collection was collected on sawdust.
The post-operative collections were collected first thing in the morning, before a meal, from the bile that accumulated overnight.
For patients who had been recently diagnosed (<9 months before data collection), data were collected on the first 12 weeks of management only ('initiation phase').
For patients diagnosed more than 3 years before data collection, data were collected on the most recent 3 years of management ('maintenance phase'), while patients diagnosed 9 months to 3 years before data collection provided data on both the initiation and maintenance phases.
With regard to the timing of sample collection, 44 of the 65 patients with M. pneumoniae had already been treated with antimicrobial agents before nasopharyngeal specimens were collected.
Following standard urine collection protocols, urine specimens were collected by each participant in clean, dry, nonreactive plastic containers that we provided the night before.
The modal age at sample collection was 25 hours and 95% of samples were collected before 4 1/3 days of age.
Five collections of gene sets were collected.
The observers spent a period of 1 month in training and orientation to the data collection methods before any real-time data were collected.
Consent forms signed by both students and one of their guardians were collected before the baseline data collection.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com