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Digestion was stopped before collection of cell suspensions.
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For all treatments, cells were cultured in 24 well plates before collection of supernatants and cell analysis.
At subconfluency, the growth medium was changed with quiescent medium (serum free) 24 h before collection of conditioned media (CM) and cell lysates.
Retrovirus was packaged using PG13 cells (European Collection of Cell Cultures, Porton Down, UK).
AR42J cells (rat pancreatic adenocarcinoma) were obtained from ECACC (European Collection of Cell Cultures, Salisbury, UK).
DLD1 (colon adenocarcinoma cells) obtained from European Collection of Cell Cultures Salisbury UK (ECACC™).
Collection of cell samples.
Patients who had been treated with chemotherapy during last 4 weeks before collection of ascites showed poor percentage of cancer cells in ascites and no significant correlation between IL-8 and angiogenesis index was found.
To visualize luciferase expression in the cancer cells, the anesthetized mice received d-Luciferin before collection of the bioluminescence images.
After complete removal and washout of the agonist, cells were bathed in PBS for 2 min before collection of samples of BzATP-induced ATP release.
To explore the associated mechanisms, Huh7 cells were treated with the ERK inhibitor Pd98059 or transfected with AhR siRNA before collection of the medium.
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