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Hospital staff trained in the study protocols explained the study and obtained consent before collection of data, and collected blood samples from mothers at time of delivery.
Before collection of water in a particular bottle, the bottle is rinsed thoroughly with the respective samples of the groundwater.
Before collection of the DRIFT spectrum, nitrogen was purged through the beam path at 14 L min−1 (NTP).
The tap was allowed to run for few minutes; the container was rinsed three times with greywater before collection of the final sample.
The bottles were rinsed three times and taps were allowed to run for at least 5 min before collection of samples and labelled accordingly.
All women provided written informed consent before collection of the samples.
For all treatments, cells were cultured in 24 well plates before collection of supernatants and cell analysis.
For three days before collection of a 40 ml blood sample, all participants refrained from using all medications, including β2-adrenergic agonists and H1-antihistamines.
All patients provided informed consent before collection of specimens.
Informed consent is obtained before collection of any data.
Digestion was stopped before collection of cell suspensions.
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