Sentence examples for before cells were used from inspiring English sources

Label incorporation was determined to be >95% by MS before cells were used for further experiments.

Before cells were used for confocal microscopy, cells were harvested by centrifugation and resuspended in fresh SC-glucose medium.

Aliquots of cells were incubated with fluo-4 AM (10 μM) for 20 min, and this mixture was allowed to de-esterify for at least 30 min before cells were used.

Breast cancer cells stably over-expressing RASSF1C were incubated for 14 days in presence of 1 μg/ml doxycycline before cells were used to isolate genomic DNA for DNA fragmentation analysis using an Apoptotic DNA Ladder Kit (Roche Applied Science, Mannheim, Germany).

Type I cells were plated on 15 mm round poly-d-lysine-coated (0.1 mg ml−1) glass coverslips (Warner Instruments, Hamden, CT) and incubated at 37 °C in 5% CO2, 10% O2 ~2 h before use; cells were used for experiments within 8 h of isolation.

Before OP9 cells were used as feeder cells, they were irradiated with γ-rays (50 Gy).

Twenty four hours before the cells were used in the experiments, the cells were sub-cultured at 2×106 cells/mL in 25-cm2 flasks with fresh culture medium.

Pure acentriolar cell lines were established through clonal propagation from single cells, a process taking 4 5 weeks, before these cells were used for experiments.

Melanoma cells were infected with 1 × 10 IU for 24 h using the calcium phosphate method and the medium was replaced two times before the cells were used in different assays.

Pure acentriolar cell lines were further isolated through clonal propagation from single cells, a process taking additional four to five weeks, before these cells were used for reconstitution experiments (see b) below).

Pure acentriolar cell lines were subsequently established through clonal propagation from single cells, a process taking additional 4 5 weeks (before these cells were used for experiments), generating a number of independent SAS-6 -/-; p53 -/-cell lines all behaving similarly (with clone #1 and #2 being characterized here).