Sentence examples for before cells were treated from inspiring English sources

Fibroblasts were plated at 70% confluence in 6-well plates for FACS analysis or for immunocytochemistry purposes on 12-well diagnostic microscope slides (Cel-Line, Thermo Scientific, Braunschweig, Germany) and allowed to adhere for at least 6 hours before cells were treated.

Therefore, in the following experiment we added geniposide to the cell culture medium before cells were treated with formaldehyde.

The medium was then replaced with serum-free DMEM/F12 overnight before cells were treated with C. trachomatis or TLR2 ligand, as described above.

Preincubation with JNK inhibitor, SP600125 (40  μM), or/and ERK inhibitor, PD98059 (60  μM), was carried out for 30 min before cells were treated with 50  μM FK506.

After 32 h for LNCaP and 24 h for PC-3 cells the medium was changed to serum-free RPMI 1640 and Ham's F12 K medium, respectively, for 24 h before cells were treated with the indicated reagent.

Fresh media was added 18 24 hours following transduction and cells were cultured for a further 2 days before cells were treated with osteogenic media with 200 ng/ml BMP-2 for another 3 days.

Twelve hours before collection, cells were treated with 20 μmol/L of MG132 (Sigma-Aldrich, USA).

A total volume of 200 µL VK2 cell suspension was seeded into each well of a 96-well microtiter plate and placed into a humidified atmosphere containing 5% CO2 at 37 °C for 24 h before the cells were treated.

Fibroblasts were plated at 70% confluence in 6-well plates and allowed to adhere for at least 6 hours before the cells were treated with varying doses of curcumin for 72 hours.

Before harvest, cells were treated with the proteasome inhibitor MG132 (Millipore) for 4 hr.

At 2 h before collection, cells were treated with EdU, a thymine analog, to allow for incorporation in replicating cells.