Sentence examples for before cells were seeded from inspiring English sources

Following, fresh media was added, and the plate was kept in a humidified incubator (37 °C, 5% CO2) for at least 12 hours before cells were seeded.

Before cells were seeded, the fibronectin solution was exchanged for cell culture medium and heated for 30 minutes in the incubation chamber of the microscope at 37°C in order to adjust to the higher temperature.

For migration assays silicon spacers were inserted into 12-well plates before cells were seeded.

For the invasion assays, matrigel (BD Biosciences) was added to the transwell chambers and incubated for 5 h before cells were seeded.

The cell invasion assay was carried out similarly, except that the matrigel (BD Biosciences) was added to each well 6 h before cells were seeded on the membrane.

Briefly coverslips were placed in 6-well plates and an insert (ibidi, Munich, Germany) was placed onto the coverslip before cells were seeded.

The day before treatment, cells were seeded in 6-well plates at 500 or 1000 cells per well, dilutions per dose, and three replicates per condition.

After an hour the media was replaced and returned to the incubator for at least 12 hours before the cells were seeded.

Before the cells were seeded, the heat-treated porcine bone blocks were divided into two groups: collagen coated blocks (n=16) and uncoated blocks (n=16).

For siRNA transfection, 24 hours before transfection, cells were seeded into six-well plates at a concentration of 2×105 cells/ml to obtain 30 60% confluence at the time of transfection.

One day before transfection, cells were seeded in six-well plates with a concentration of 105 cells/well.