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We transfected HeLa cells with BRCA1 or siCtrl, and treated with a minimal effective dose of bortezomib (10 nM) for 20 h before cells were processed for cell cycle analysis by the fluorescence-assisted flow cytometry.
After this viral adsorption period, the inoculum was removed, the cells washed once with PBS, and fresh culture media without SFB was added to complete 10 (RRV) or 12 (Yuc8) hpi before cells were processed by immunocytochemistry (See below).
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After the incubation period, cells were processed as described above before analysis on the FACSCanto flow cytometer.
Cells were processed immediately after isolation.
Individually, a cell is processed asynchronously relative to other related cells and is queued before being multiplexed over the transmission path.
Cell lysates were processed for Western Blotting.
Cell pellets were processed for SDS-PAGE.
For TMT quantitation, cells from each condition were processed separately, and peptide samples were labeled with TMT reagents before pooling.
Images were processed using Cell-M software.
Indicate how samples were processed before storage.
The swabs were processed as before.
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