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This was repeated five times before cells were plated for titer.
Viability was determined by trypan blue exclusion, before cells were plated.
The cover slips were rinsed twice with PBS before cells were plated.
Cells were then maintained without doxycycline for 2 3 passages before cells were plated and used for experiments.
The polymerized collagen gels were washed with 1 mL epithelial cell growth medium before cells were plated and grown overnight.
For cell invasion experiments, Matrigel was plated inside Transwell culture inserts for 5 h at 37°C before cells were plated onto the inserts (50,000 cells/well).
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Twenty-four hours before transfection, cells were plated onto 100 mm plastic dishes in medium supplemented with charcoal stripped and dialyzed FBS (SD-FBS).
Pre-incubation with PTX, the functional blocking anti-CD47 antibody or the COX-2 specific inhibitor NS-398, before the cells were plated onto collagen I, produced a similar rounded cell morphology and a homogenously dispersed CD47/α2 integrin staining pattern (Fig. 4A).
Before transfection, cells were plated on the appropriate dishes.
One day before transfection cells were plated into the required growth plates.
One day before transfection, cells were plated in 24-well plates at 50% confluence.
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