Sentence examples for before cells were passaged from inspiring English sources

To further address this point, we cultured keratinocytes with different inhibitors for 3 days before cells were passaged at clonal density.

Proliferating cells were passaged before reaching confluence.

Cells were passaged before reaching 70 80% confluence.

The cells were passaged before reaching confluence and used at between five and six passages.

Cells were passaged before they reached the confluence at a ratio of 1 : 3–1 : 6 according to their proliferating properties over time and at regular intervals.

Before harvesting RNA, human ES cells were passaged on Matrigel (BD Biosciences) or fibronectin (Sigma -coated plateSigma -coatedd in human ES medium conditioned with inactivated MEF [ 36] for at least an additional 3 plateses.

Cells were passaged twice before integration of the lentiviral vector was confirmed by PCR using genomic DNA of transduced cells as template.

The medium was changed every day, and the cells were passaged by trypsinization before confluence.

The supernatant was plated and cells were passaged 4 times before those with the genotype SmnC-T-Neo/+ CreEsr1 were uSmnC-T-Neo/+ CreEsr1 SmnC-T-Neo/+ CreEsr1

Cells were passaged the day before the assay was performed.

The cells were passaged 24 h before transfection.