Sentence examples for before cells were lysed from inspiring English sources

Particle incubation was then continued for further 20 h before cells were lysed, seeded on Lysogeny broth agar plates and incubated for 6 days.

This reaction was quenched with 1 M Tris-HCl pH 7.5 before cells were lysed in RIPA for 30 min on ice with occasional agitation.

(B ) As before cells were lysed and immunoblotted with the indicated antibodies following infection with Listeria strain EGD-e PrfA* (MOI 10), ΔplcA (MOI 10), Δhly (MOI of 10), and a triple mutant of ΔhlyΔplcAΔplcB (MOI of 100).

Site-directed mutagenesis was carried out by means of PCR with the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies, Stratagene Products Division, La Jolla, CA, USA).. Freshly transformed E. coli Topp3 cells were induced with 0.5 mM IPTG, and the CYP3A4 construct was expressed for 48 72 h before cells were lysed by sonication; expression levels were quantified by reduced CO binding.

Ligand stimulation was carried out using 4 n M EGF, 5 n M TGF α or 100 n M HRG for 10 min at 37°C before cells were lysed in RIPA lysis buffer (50 m M Tris-HCl, 1% NP-40, 0.1% SDS, 0.25% sodium deoxycholate, 150 m M NaCl, 1 m M EDTA).

'RNAprotect Bacteria Reagent' (QIAGEN, USA) was used to stabilize RNA before bacterial cells were lysed.

After the infection, the cells were gently washed once, before the 293 cells were lysed by addition of 1% saponin in PBS for 15 min. Cell-associated and intracellular bacteria were suspended by vigorous pipetting, and colony forming units were determined by plating of serial dilutions on GC agar.

For the splenocytes, the red blood cells were lysed and washed before the following steps.

Spleens, thymi and lymph nodes were removed from 2 4-month-old mice, mashed in cell strainers and disaggregated, and red blood cells were lysed as required before the lymphocytes were suspended in RPMI 1640 medium containing L-glutamine (Invitrogen) with 10% (v/v) heat-inactivated FBS (fetal bovine serum), 50 units/ml penicillin, 50 μg/ml streptomycin and 50 μM 2 4-month-oldanol.

Spleens, thymi and lymph nodes were removed from 2 4-month-old mice, disaggregated and red blood cells were lysed as required before the lymphocytes were suspended in RPMI 1640 medium containing L-glutamine (Invitrogen) with 10% (v/v) heat-inactivated FBS (fetal bovine serum), 50 units/ml penicillin, 50 μg/ml streptomycin and 50 μM 2 4-month-oldanol.

Briefly, cells were lysed overnight at 37°C before adding protein precipitation solution.