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In addition, spontaneous release was measured before cells were exposed to HS to quantify the release rate at 0M sucrose.
When pre-treatment of cells was required, 5 mM N-acetyl l-cysteine was applied to V79 cells for 24 h before cells were exposed to DETC.
However, before cells were exposed to the hypoxic condition, they were treated under normoxic condition for 1 h then maintained under hypoxic conditions for 16 h.
Exponentially growing cells were seeded in 6-well plates overnight to allow attachment before cells were exposed to a range of concentrations of drugs for 24 h.
Fresh media with or without metformin were added just before cells were exposed to either normoxia (21% O2) or hypoxia (1.5% O2) for 16 hr.
SKBR3 cells were preincubated alternately at pHe 7.4 or pHe 6.8 for 30 min, before cells were exposed to medium containing prolactin or vehicle for 15 min at either the same pHe or the alternate pHe, and prolactin-induced Stat5, Jak2 and Erk signals were examined.
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(3) Before cells are exposed to osmotic stress, we assume that the system is in a steady state, which means that the Hog1 distribution in cytoplasm and nucleus, the glycerol and the Pbs2 phosphorylation level are constant before osmotic stress stimulation.
GO or S-rGO suspensions were freshly prepared before the cells were exposed and diluted to appropriate concentrations from 20 to 100 μg mL−1 with the culture medium; they were then immediately applied to the cells.
The cytokine was perfused for 5 min before the cells were exposed to hypoxia.
Before harvesting, cells were exposed to 10 μM BrdU for one hour.
The truncates, as illustrated in Figure 5A, upper panel, were transfected into HEK293T cells before the cells were exposed to MG132 and H2O2 treatments.
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