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Both cultures were grown for 6 h before cells were collected using centrifugation.
Plates were incubated for 48 h before cells were collected for further analysis.
Plates were incubated for 48 h at 37 °C before cells were collected and subjected to further analysis.
Golgistop was added in the presence of phorbol-12-myristate-13-acetate phorbol-12-myristate-13-acetate phorbol-12-myristate-13-acetate phorbol-12-myristate-13-acetate phorbol-12-myristate-13-acetate
Cells were serum starved then treated with rcIL-6 for 0, 5, 10, or 30 minutes before cells were collected for Western blotting.
For MDA-MB-231 study, cells were left untreated or treated with the gamma-secretase inhibitor (GSI) Compound E (Axxora, Farmingdale, NY) at 10 μ M for the times noted, before cells were collected, and analyzed as described earlier.
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Cells were then transferred to ice, and washed twice with ice-cold PBS before being collected.
The plates were incubated for an additional 24h before the cells were collected.
To stain membranes, 0.5 μl of FM4-64 (100 mg/ml) was added to samples just before the cells were collected by centrifugation.
The cells were stimulated with 200 U/ml IFN-γ for 24 h before supernatant and cells were collected.
In the DI-EC regimen, peripheral blood progenitor cells were collected before chemotherapy.
More suggestions(12)
before larvae were collected
before tissues were collected
before nuclei were collected
before cells were harvested
before eggs were collected
before cells were used
before cells were analyzed
before cells were exposed
before samples were collected
before solids were collected
before supernatants were collected
before data were collected
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