Sentence examples for before cells were analyzed from inspiring English sources

As before, cells were analyzed for expression of CD40, CD80, CD86 and ICAM-1 by flow cytometry.

Propidium iodide (PI) (1.4 μg/ml) was added 5 min before cells were analyzed using an LSRII flow cytometer (BD Biosciences, San Jose, CA, USA).

Briefly, 5 × 10 target cells were incubated for 2 h with isolated NK cells at a ratio of 1 1 in the presence of 0.1 μg of an allophycocyanin-conjugated CD107a antibody (1D4B; Southern Biotechnology Associates, Birmingham, AL, USA) before cells were analyzed by flow cytometry.

For intracellular labeling with anti-H4R and anti-cyclin D3 antibodies cells were treated with FACS permeabilizing solution from BD before staining Cells were analyzed in a FACSCanto II cytofluorometer (Becton Dickinson, Mountain View, CA), using FlowJo software.

Approximately one minute before the cells were analyzed, propidium iodide (Sigma) in PBS was added at a final concentration of 10 μg/mL.

After transfection with siRNA, the cells were grown for three days before infection with fluorescent DsRed-expressing Salmonella typhimurium and cultured for another 18 h before the cells were analyzed for intracellular (fluorescent Ds-Red expressing) bacteria by flow cytometry.

Before the cells were analyzed on day 12-14, dead cells were removed by Ficoll-gradient centrifugation resulting in a > 98% pure T cell population as judged by CD3 FACS analysis.

To exclude dead cells, 5 ng propidium iodide (PI; Sigma) was added just before analysis and cells were analyzed using a FACSCalibur cytometer and CellQuestPro software.

To explore this, early passage cultured aortic smooth muscle cells were analyzed before and after stimulation with TGF-β1 and ET-1, which has been shown to induce an overlapping cohort of profibrotic genes in other cell types [ 22].

Accordingly, the surface markers CD90, CD73, and CD105 were used for positive hMSCs identity and CD45, CD34, CD19, CD11b, and HLA-DR were used as negative markers. 25 Cells were analyzed before and after 5-days incubation in the 3D gels, using standard flow cytometry protocols.

The type and distribution of stained cells were analyzed before cell counts were performed.