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The first measurement series was performed on intact branches in the morning immediately before branch cutting.
E and gL exhibited greater (P < 0.05) values in H treatment both before branch cutting in the morning and under moderate water deficit (ΨL≥-1.55 MPa).
Use of unbranched intact Equisetum shoots (e.g. shoots of Equisetum hyemale or developing shoots of E. giganteum before branch emergence) would allow for likely greater accuracy in studying whole-plant water relationships without the need for extreme destructive manipulation to measure exudation rates.
We acknowledge that data from such field experiments do not allow strict separation of the rapid and long-term effects of water deficit, however, this approach was encouraged by absence of differences both in ΨL and ΨB between the treatments before branch cutting in the morning (see Table 2).
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