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Before bone marrow cell seeding, GRHC pellets were sterilized by autoclaving (120°C, 20 min).
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and their femurs isolated before bone marrow cells were flushed out with supplemented DMEM (10 % FBS, and 100 U/mL penicillin and 100 mg/mL streptomycin).
BMC: bone marrow cell.
To examine the role of cell-to-cell interaction between tumour cells and host cells in osteoclast formation, we fixed MDA-231 cells before the contact with bone marrow cells.
The proportion of CD45high to CD45low cells was different before and after cultivation of bone marrow cells.
There was no difference in the total number of bone marrow cells before and after Rapamycin treatment.
Immunocytochemistry and RT-PCR confirmed that some adherent cells differentiated into smooth muscle-like cells that expressed α-SM actin under these conditions, unlike the bone marrow cells before culture.
Therefore, tentative diagnosis of malignant lymphoma was made based on flow cytometry of bone marrow cells before the final diagnosis by lymph node biopsy in about 50%% of LAHS patients.
Bone marrow transplantation is relatively painless for the donor, but the recipient needs to be close to death since the procedure requires that their native bone marrow cells be killed before the new cells are transferred intravenously and migrate to the bone.
For analysis of surface markers CD11b (cluster of differentiation 11b) and EMR1 (epidermal growth factor-like module-containing mucin-like hormone receptor-like 1), native bone marrow cells were collected before erythrocyte lysis.
BAPTA-AM was incubated with fresh bone marrow cells for 1 h before stimulus with ATP.
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