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Cooks often let them dry for a day or two before boiling them in very salty water.
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Mr. Calias puts lobsters in a freezer for a few minutes before boiling them, to anesthetize them.
Bagels also have a chewy, shiny crust, achieved by boiling them in water before baking.
Just make sure to boil them in boiling water so that they become sterilized before you place them in the tank.
Crack acorns and boil them in water.
Puffballs were prepared by drying them in the sun, grinding them with a mortar, and boiling them before eating with acorn soup.
Place them in the refrigerator the night before you plan to boil them.
A minute or so before boiling whisk in the egg mix.
Precipitations were washed 5 times in lysis buffer before boiling in Laemmli buffer.
Briefly, samples were reduced in 10 m M dithiothreitol (DTT) and alkylated in 50 m M iodoacetamide before boiling in loading buffer, and then separated by one-dimensional sodium dodecyl sulphate (SDS)–PAGE 4 12% Bis-Tris Novex mini-gel from Invitrogen (Paisley, UK) and visualised by colloidal Coomassie staining from Invitrogen.
Immunocomplexes were precipitated using protein A-sepharose beads for 2 h and were washed five times with extraction buffer before boiling in sodium dodecyl sulfate (SDS) sample buffer.
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