As before, bi-dimensional genome-wide levels of significance were calculated using a Bonferroni correction assuming statistical independence every 30 cM.
Their application was filed before BI's, but because the BI group sought an expedited review process, it was awarded the key patents first.
Such irregular metaphase-anaphase transition, before bi-orientation of all chromosomes, would readily explain our findings of unsynchronized sister-chromatid separation, a high frequency of non-disjunction, and diplochromosomes in metaphase spreads.
These findings contribute to explain how initial end-on kinetochore-microtubule attachments are stabilized before bi-orientation.
The specific, in vivo cytotoxicity of Bi-PAI2 against the uPA receptor was directly tested by preinjection of increasing concentrations of PAI2, before Bi-PAI2 administration.
PCR products were cleaned before bi-directional dideoxy sequencing and sequence traces analysed using the Staden Package suite of programs (http://staden.sourceforge.net/, accessed 26 July 2010).
To investigate which factors are responsible for kinetochore-microtubule attachment stability before bi-orientation, we established a system in Drosophila S2 cells undergoing mitosis with unreplicated genomes (SMUGs) (Drpic et al., 2013).
We then left the micropipettes in air for 2 4 h before silanizing them with bis(dimethylamino dimethylsilane (Fluka 14755) as described before [16].
Twelve fish (3 per tank) were sampled for plasma cortisol before the temperature increase (BTI), and compared with values before injection (BI), to allow us to determine the degree of stress induced by the increasing temperature regimen and being held at 16°C for 1 week.
Bis means encore, again, more; and when put before cotto (cooked), bis means again, or twice, so biscotto means twice-cooked.
