Sentence examples for before being washed again from inspiring English sources

Later, they were re-treated 12 hours before being washed again.

Membranes were subsequently washed with TBST and incubated for 1 h at room temperature with HRP-conjugated secondary antibodies before being washed again.

After washing, samples were incubated for 30 min with secondary FITC-conjugated antibodies (Sigma F1010 & F9259) at 1 100 before being washed again.

Sections were probed with primary antibodies (dilutions and manufacturer details in Additional file 3) for 1 h at 20 °C before being washed again.

Membranes were washed three times with TBST and were incubated 1 h at room temperature with horseradish peroxidase-conjugated secondary antibodies before being washed again three times with TBST.

After three 3-min washes in PBS/Tween 20 buffer (wash buffer), sections were incubated in 1% glycine for 30 min to neutralise acidic groups before being washed again in the same wash buffer.

Alkaline phosphatase-labeled goat anti-human polyvalent IgM, IgG, and IgA (50  μL) (Sigma-Aldrich, St . Louis MO) diluted 1 : 1,000 were added before plates were washed again 5 times with PBS-Tween.

The sampling bottles were soaked in 1 1 diluted HCl solution and washed twice with distilled water before sampling and were washed again in the field with groundwater sample filtrates.

Immediately before stimulation cells were washed again with medium again and cells were stimulated with 2 ng/ml IL-1β (Miltenyi Biotec) or 25 ng/ml Flagellin (Invivogen) for 6 hours and supernatant was collected and stored at −20°C for ELISA.

Cells were washed again as before and then counterstained with Hoechst 33342 for 2 min. Cells were washed with and imaged in PBS.

The plates were washed again as before, and the antigen-antibody reaction was detected by incubation with HRP-conjugated goat anti-mouse IgG (1 : 1000) for 2 h at 37°C.