Sentence examples for before being transfected using from inspiring English sources

SW480 cells were seeded grown to 50% confluent under the standard conditions for 72 h, before being transfected using Lipofectamine 2000, as per the manufacturer's instructions.

Cells seeded onto 24 well plates (1 × 10 cells/well) the day before were transfected using 0.3 μg pcDNA3-IR or pcDNA3 (empty vector, EV) and 0.05 μg pAP1-Luc.

Cells seeded onto 24 well plates the day before were transfected using 0.3 μg pcDNA3-IR-B (HeLa cells) or EV (HEK293 cells), 0.05 μg pAP-1-Luc and different amounts of Mut.

In LOF studies, cells were seeded to 30% confluency before siRNAs were transfected using Lipofectamine RNAimax (invitrogen) at a concentration of 20 µM.

CHO cells were transfected using ExGen500 (Eurogentec).

For cell death assay on DNA transfected cells, the cells were split at 1 15 ratio 16 hours before transfection and were transfected using Jetprime reagent (about 40 50% confluency during transfection).

For DNA transfection, the cells were split at 1 5 ratio 16 hours before transfection and were transfected using Jetprime reagent.

Melanophores were transfected using the FuGENE6 transfection reagent (Roche Diagnostics).

siRNA and mRNA reporters were transfected using Lipofectamine 2000.

siRNAs were transfected using lipofectamine 2000.

Cells were transfected using FuGENE HD (Roche).