Sentence examples for before being terminated by from inspiring English sources
ERS-1 operated for almost nine years before being terminated by the European Space Agency owing to failure in its attitude control system (ACS); no report about the cause of the ACS failure was prepared.
Kinase reactions were incubated 30 minutes at room temperature before being terminated by the addition of gel loading buffer.
Labeling reactions were incubated for 30 min at ambient temperature before being terminated by addition of reducing sample buffer and heating (70 °C, 10 min).
Reactions were incubated at 30°C for 2 h before being terminated by the addition of reducing SDS loading buffer and denaturation at 95°C for 4 min. Samples were analysed by western blotting using an anti-PCNA antibody.
The reaction was started by the addition of ATP (2 mM final) and reactions were incubated at 37 °C for 10 min before being terminated by the addition of an equal volume of stop buffer.
Then, Scc1-HA was induced for 1 h in the other culture (noc-loaded) before induction was terminated by shifting to glucose.
The S296 vector was electroporated into the DH10B bacteria containing the RP23-79E22 Bandand pSV1.RecA plasmid and allowed to grow overnight before recombination was terminated by growth at 43°C.
After 1 week of recovery, these mice received daily subcutaneous injections with 100 μg/kg DES dissolved in olive oil or the olive oil vehicle alone for 1 week before they were terminated by cardiac puncture under isoflurane anesthesia.
Agonists were then added for the times indicated (0.1 nM–3 µM, triplicate wells) before incubations were terminated by fixation with 3% paraformaldehyde in phosphate-buffered saline (PBS, 10 min at 21°C).
dissolved in olive oil or the olive oil vehicle alone for 1 week before they were terminated by cardiac puncture under isoflurane anesthesia after which the uterus was weighed (n = 6 mice per group).
