Talk about being synchronized.
The cultures, before testing, were synchronized by treatment with 5% d-sorbitol with a parasitemia of 0.6 0.8%.
Anaesthesia was continued with 1.5% Isoflurane and the animal was carefully observed for a variable time period (10 15 minutes) to ensure rhythmic and automatic breathing, before, the MR scanner was synchronized to the respiratory cycle (ECG Trigger Unit HR V02, RAPID Biomedical, Rimpar, Germany) by placing the button sized air-cushion sensor below the diaphragm.
With parallel technology, data had to be synchronized before it was processed.
The gas analyzer was calibrated with standard gases before each session and was synchronized with the accelerometer.
Before each experiment, cells were synchronized in phenol red-free serum free media (PRF-SFM) for 24 h.
Before the assay, cells were synchronized by 24-h culture without FBS, and then cultured in complete medium for 20 h.
Before each period, the estrous cycle was synchronized, and on Day 6 or 7 of the ensuing cycle, the time at which it was most likely to have a dominant follicle, 100 μg of gonadorelin, 25 μg of lecirelin, 50 μg of lecirelin, or 10 μg of buserelin was administered to the cows.
We next analyzed gene expression in MCF-7 cells that had been synchronized by nocodazol before check-off and further growth (7 hours) to reach G1 (FACS, data not shown).
Cultures were synchronized 24 hr before experimentation using 5% w/v sorbitol (Lambros and Vanderberg, 1979).
