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The inverse-PCR products were purified by gel extraction using the QIAquick Gel Extraction Kit (QIAGEN) before being sequenced as above.
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Clones (2 5 for each original DNA sample) were sequenced as before and the results analysed using BiQ Analyser software (http://biq-analyser.bioinf.mpi-inf.mpg.de/) (Bock et al, 2005).
24 25 If MRSA was found, the spa-gene was sequenced as described before.
DNA was sequenced as previously described [24].
Plasmids were sequenced and analyzed as before.
As before, most regions were sequenced with at least two primers on each strand.
Chromosome III, which had been sequenced before any other chromosomes as a pilot project from DNA libraries prepared from four different S288C-derivative strains (AB972, XJ24-24a, A364A, DC5), was completely resequenced in the late 1990s from strain FY1679 by the laboratories of G. Volckaert and G. Valle, who submitted the sequence to GenBank/EMBL but did not otherwise publish the revision.
The DNA insert was sequenced before the plasmid was used as the template for the following PCRs.
The results shown in Table 1 are based on analysis of individual metagenomic sequence reads ranging from 100 to 700 nt in length; the entire DNA-A would need to be sequenced before these viruses could be classified as novel strains and/or species.
Products were processed and sequenced as before.
Recombinants were sequenced before use.
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