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Calluses were transferred to 100 μM MJ-containing medium and allowed to grow for 5 days before being sampled for analysis.
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After instrumentation, a 30-min equilibration period was included before blood was sampled for baseline values of plasma glucose and plasma insulin.
Before the sacrifice, mice were sampled for blood.
All of the 12 areas were sampled for Odonata during June 2010, before the restoration (two areas were sampled twice before restoration) (for detailed sampling scheme see Additional file 1: Table S1 in Supporting Information).
and had been growing for 50 years before soils were sampled and analyzed for C and N concentrations, particle size distribution, and bulk density.
Water from fifteen wells were sampled before and after monsoon for analysis of chemical properties.
Sixty freshly harvested fruits were sampled before treatment and used for 0 day assessments.
Perfusate was sampled before and hourly after treatment for six h.
Before experiment, fish were randomly sampled for the examination of bacteria and megalocytivirus in blood, liver, kidney, and spleen as reported previously [ 31, 32].
The ensemble is then validated and tested using different data samples before being used for classifying new samples by combining the outputs of the models by simple majority or weighted voting.
The enriched culture was stored at 7 °C before being used for sample inoculation.
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