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During this tussle Booker pounded Cena's head into the ring steps before being reversed into them himself.
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DNase-treated RNA samples were evaluated on the 2010 Agilent Bioanalyzer (Santa Clara, CA) before being reverse-transcribed by the SuperScript III First-Strand Synthesis System (Life Technologies).
The H226 or primary MM cells were plated at 2.5 × 10 cells/well and 5 × 10 cells/well, respectively, in a 96-well plate before being reverse-transfected with 25 nM siRNA, as per manufacturer's instructions (Silencer Select siRNA, Applied Biosystems, Life Technologies).
Layoffs would be necessary, so the wave of hiring that had brought in new personnel only weeks before was reversed.
All material collected (due to the little number of total cells obtained from a single cloning) was firstly treated with Dnase and removal reagents (Ambion, USA) before being reverse-transcribed using Superscript II reverse transcriptase (Invitrogen).
Packaged RNA from the virion pellets were extracted with TRIzol reagent (Invitrogen) before being reverse transcribed with random hexamers and Superscript III MMLV RT (Invitrogen) according to the manufacturer's instructions.
Total RNA was isolated from shoots with TRIzol (Takara), and was treated with DNaseI (NEB) before being reverse transcribed.
RNA samples were treated with RNase free DNAase to eliminate genomic DNA contamination before being reverse-transcribed into cDNA.
To prevent amplification from possible genomic DNA contamination, the RNA was treated with DNase before being reverse transcribed.
The RNA was then treated with DNaseI (Invitrogen) to eliminate possible genomic DNA contamination before being reverse transcribed with the SuperScript RNase H- Reverse Transcriptase (Invitrogen).
Total RNAs from A. suum testis and vas deferens were isolated before being reverse transcribed into cDNA using the SuperScript III First-Strand Synthesis System (Invitrogen).
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