Sentence examples for before being quantified using from inspiring English sources

Precipitated RNA pellets were washed in 75% ethanol and re-suspended in distilled RNAse-free water before being quantified using a NanoDrop ND-1000 spectrophotometer (Labtech International, East Sussex, UK).

Cells were treated for 6 h with 5  μg/ml tunicamycin or vehicle only (100% DMSO) and then extracted using the Steady-Glo luciferase assay system (Promega) before being quantified using the Glomax 96 microplate luminometer (Promega).

DNA samples were quantified using a spectrophotometer (260 nm), and diluted in Te buffer (0.1 mM EDTA, 10 mM Tris HCL, pH 8.0) to a target concentration of 100 ng/μl, before being quantified using fluorimetry (Picogreen® dsDNA quantitation reagent Cambridge Bioscience, U.K) and diluted further to a target concentration of 50 ng/μl (± 5%).

Air-dried filters were transferred into a 24-well sample plate, covered with 1 ml scintillation fluid and incubated over night before radioactivity was quantified using a microplate liquid scintillation counter (1450 MicroBeta TriLux, Wallac).

Monitoring and analysis of trace contaminants such as pharmaceuticals and pesticides require the preservation of the samples before they can be quantified using the appropriate analytical methods.

Per A. nidulans strain, a core (1 cm diameter) from four independent plates were excised and each core was homogenized in 3 mL H2O containing 0.01%Tween-80Tween-80 spores spores were quantified using a hemocytometer.

Per strain, center cores (1 cm diameter) from three independent plates were excised and each core was homogenized in 3 mL H2O containing 0.01%Tween-80Tween-80 aspores spores were quantified using a hemocytometer.

Furthermore, changes in retinal thickness before and after surgery can be quantified using optical coherence tomography (OCT) [ 7, 8].

Evidence for atelectasis on CXR was quantified using a CXR score before and within 24 hours of treatment with rhDNase.

Viruses were pretreated with 2 U/mL DNase I (Life Technologies) at 37 °C for 30 min before infection, and the viral titer was quantified using the p24 ELISA assay (PerkinElmer, Waltham, MA, USA).

Fish species richness and abundance was quantified using electrofishing surveys before (4 sampling trips) and after (3 sampling trips) SWD addition.