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The squashed specimens were further cleaned using 1X SSC to remove the cellular debris, before being imaged using AFM.
The phantoms were stored in resin frames to protect them and keep them flat, but were removed from the frames before being imaged using a range of modalities, as described below.
Lower pharyngeal jaws were extracted and cleaned in a solution of 10% trypsin for 17 h before being imaged using a desktop scanner.
After incubation, cells were rinsed with PBS, the nucleus of cells were fluorescently stained, and the cells were fixed before being imaged using an OperettaTM High Content Imaging Systems instrument.
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Patients were imaged using US before and after contrast agent dosing at baseline and again at weeks 6, 12, 18 and 24 after treatment started.
Instead, water protons in the blood are used as the tracer and are labeled by inversion pulses before they are imaged using a fast proton density weighted imaging sequence (echo planar imaging or fast spin echo sequence).
The mounting medium was allowed to dry before the slides were imaged using a Leica PS2 confocal microscope equipped with an Argon 488 nm gas laser and Helium Neon lasers (543 and 633 lines).
In this platform, embryos are embedded in plastic and serially sectioned, before each block face is imaged using a high-resolution digital camera.
The sample was imaged using fluorescence and lens-free imaging to acquire "before" images.
To determine a starting value for the workload during the exercise test, participants initially performed a 30 second maximal exercise using the ergometer before being imaged.
The spent medium was removed, and the cells were washed with PBS three times before being imaged on a confocal laser scanning microscope, using 488 nm excitation and fluorescence detection over 600 630 nm.
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