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The resulting gels were removed, dried and exposed to Molecular Dynamics phosphor screens before being imaged on a Storm 720 scanner.
The spent medium was removed, and the cells were washed with PBS three times before being imaged on a confocal laser scanning microscope, using 488 nm excitation and fluorescence detection over 600 630 nm.
Serial 10 μm frozen tissue sections were cut, and slides fixed in ice cold acetone for 10 min before being imaged on a fluorescence microscope for Hoechst 33342 uptake.
For immunofluorescence, serial 10 μm frozen tissue sections were cut, and slides fixed in ice-cold acetone for 10 min before being imaged on a fluorescence microscope for Hoechst 33342 uptake.
Cells were incubated for 24 48 h at 37°C before being imaged on a CKX41 microscope (Olympus, Center Valley, PA, USA), and photographed using a DP20 camera (Olympus).
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Next, the coverslip was placed on the slide, and FluorSave reagent dried for two hours to overnight before cells were imaged on a Zeiss (Jena, Germany) Axioplan 2 fluorescence microscope.
Mice were imaged before CTL injection and on three consecutive days after injection.
Zebrafish fins were imaged before amputation and again on day 3, 5, 8 and 14 postamputation.
When analyzing PV-interneurons in particular, the coverslips are scanned to obtain images as before but all PV-interneurons on the coverslip are imaged.
Mice were imaged before being sacrificed.
Last summer, the "cat detector" at Google (which I've mentioned before) was trained on ten million images, using a thousand machines, for three days, and if the program managed to "learn" what a cat looks like, its overall visual performance was poor.
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before being posted on
before being activated on
before being sacrificed on
before being installed on
before being issued on
before being loaded on
before being buried on
before being freed on
before being placed on
before being sold on
before being grafted on
before being analyzed on
before being commissioned on
before being tackled on
before being put on
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com