Sentence examples for before being grown in from inspiring English sources

Transformants were screened by FACS analysis before being grown in the absence of tetracycline to select cell lines with optimal control of expression.

The seeds were chilled at 4°C in the dark for 5 days before being grown in soil medium (Bio-mix TTing Substratum, Moerdijk, Nederland).

Briefly, leaf discs (0.5 cm × 0.5 cm) were dipped in Agrobacterium overnight culture (OD600 = 0.6), regenerated under kanamycin selection (30 μg/ml) and rooted plantlets hardened off before being grown in a greenhouse under conditions described above.

For clonogenic assays, cells were infected with shRNAs at low MOI in six-well plates (three per shRNA) and selected with puromycin for 3 days before being grown in drug-free media for 10 14 days.

The discrepancy may be explained by differences in the growth conditions since in the above study [ 14], seeds were germinated in light for two to six hours before being grown in the dark for three days in the presence of PAC, whereas in the study analyzed here, seeds were stratified and germinated in the presence of light and PAC or ABA [ 60].

Noguchi devised means of cultivating microorganisms that had never before been grown in the test tube.

He bought orchids that only a day or two before were growing in Thailand.

Monocyte-derived macrophages were obtained by adherence of fresh monocytes, in serum-free medium, to glass coverslips for 1 h before being grown on in RPMI supplemented with 10% fetal bovine serum (FBS), Glutamax, pyruvate, penicillin/streptomycin, non-essential amino acids and 50 ng/ml M-CSF for 6 or 7 days at which point cells were spread and strongly adherent.

From a microbiologic vantage it was not possible to ascertain if current avirulence was an attenuation effect that arose from passage in the mule before it was grown in culture and re-inoculated or if it was an effect of the in vitro culture.

The last three transfers before harvesting were grown in medium without agar.

Cells were then grown for two additional passages in medium containing HT but lacking Aminopterin, which is the component assimilated by HPRT, to allow TS cells to recover from the selection before they are grown in standard TS medium again.