Sentence examples for before being frozen at from inspiring English sources

They used a pig's brain, which was perfused with lethal glutaraldehyde before being frozen at –135C, a method called aldehyde-stabilised cryopreservation (ASC).

Samples of adult bees (∼150 bees) were removed from a central brood frame, placed into a 50 ml centrifuge tube, and temporarily stored on dry ice before being frozen at –80°C for future processing.

After harvesting, samples were stored on ice until roots were removed from the base of the tiller and tillers were cut to their previous defoliation height (20, 40 or 60 mm) before being frozen at −20°C before freeze-drying.

Samples were spun at 3000 rpm and cell pellets washed twice with water before being frozen at −80°.

These were then separated by centrifugation at 10,956 × g for 1 minute at room temperature before being frozen at -20°C until further analysis.

Serum samples collected during summer transmission periods (July September) were tested for circulating virus before being frozen at –70°C until further processing (see below).

For comparison, approximately 300μl of blood was taken from the brachial vein, transferred to RNAlater immediately and stored at room temperature for up to 12 h before again being frozen at –80 °C.

The VLP preparations were then dialysed extensively against dialysis buffer (PBS plus 400 mM NaCl) before being frozen in aliquots at -80°C until use.

Serum was collected into two 1.8-mL cryovials (Nunc. Roskilde, Denmark) and kept at 4°C in a portable gas refrigerator for up to 14 days before being frozen and stored at –20°C.

Organic solvent was removed from selected fractions under reduced pressure before being frozen, lyophilized, and stored at −20 ˚C until further use.

Adult flies were kept at this temperature for four to five days before being frozen in liquid nitrogen, and stored at −80°C.