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This is a two-phase procedure; the first stage requires the insertion of the standard 24G i.v. catheter before being flushed with 0.2 0.3 mL of 1 % Heparin solution in saline.
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Johnson had testified during the trial that he had been left lying on a gurney as long as 20 minutes at Pomona Valley Community Hospital before his eyes were flushed with water, a treatment that his attorney claimed may have spared all or part of his eyesight if it had been given immediately.
Before thermodesorption, traps were flushed with helium at 50 ml min−1 for 2 min to remove moisture and oxygen.
Immediately before sampling, each container was flushed with ambient air [21] to ensure that GHG concentrations at the headspace were at ambient levels.
Before being closed, the bottles were flushed with nitrogen gas (N2) to remove air in the flask head prior to incubation; the temperature was maintained at 35 ± 1 °C.
Before collecting filtrate, each filter was flushed with 350 mL lakewater to extract filter-derived contaminant organics, and filters were changed after filtering 3 L lakewater to minimize clogging-induced lysis or sorption.
Before each experiment glutathione powder was flushed with nitrogen and then dissolved in pH 8.0 phosphate buffer.
In brief, 10 cm of small intestine was flushed with water before being tied and everted over a glass rod.
All syringes were flushed with N2 before being used to transfer buffer, reagents, or proteins from the stock vials to the assay cuvettes.
Before sampling, the syringe needle space was flushed with another 4 mL of soil air as described above with a Luer lock system.
Before taking any measurements, the system was flushed with sterile saline and the pubic symphysis was always used as zero reference point with the subject in the complete supine position.
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