Sentence examples for before being fixed with from inspiring English sources

They were then washed in distilled water twice before being fixed with 4% paraformaldehyde for 20 min.

They were then washed in distilled water twice before being fixed with 2.5% glutaraldehyde for 20 min. The samples were washed in distilled water three times again, then air-dried for AFM scanning.

Some of the tissue-engineered bone membrane implants, which were cultured for 5 days were removed from the 6-well plaque and washed twice with PBS before being fixed with 2.5% neutral glutaraldehyde for scanning electron microscopy (SEM JSW-680LA, Japan) inspection.

Cells were washed gently with PBS before being fixed with 4% formalin for one hour.

Then the cells were washed again with PBS before being fixed with 70% ethanol at −20C overnight.

The salivary glands were incubated in chromatin wash buffer before being fixed with formaldehyde to remove any unbound Rbf1N-RFP.

Following incubation cells were washed twice with PBS, before being fixed and permeabilized with 3.7% formaldehyde and 0.2% triton x100 for 20 minutes, again at 37°C.

Cells were Fc blocked with CD16/32 and stained with FITC – or APC – conjugated CD4, CD8a, or CD8b antibodies (BD Biosciences) before being fixed and permeablized with the Cytofix/Cytoperm kit and stained with PE – conjugated IFNγ (XMG1.2, BD Biosciences).

After 24 h, cells were incubated with mouse anti-FLAG-M2-Cy3 antibody (Sigma-Aldrich Co). at 4°C and stimulated with 20 nM CCL2 at 37°C for the times indicated before being fixed, and incubated with goat anti-FLNa (Santa Cruz Biotechnology, Inc).

The cells were washed and further cultured for 24 h before being fixed and stained with Giemsa.

The cells were allowed to migrate for 6 h at 37°C and 5% CO2 before being fixed and stained with crystal violet.