Sentence examples for before being fixed and from inspiring English sources

After treatment, conceptuses were incubated in KSOM-AA medium for 30 minutes at 37°C before being fixed and processed as described in previous section.

Cells are grown and treated under standard tissue culture conditions before being fixed and printed onto replicate glass slides, effectively decoupling the experimental conditions from the assay technique.

Following incubation cells were washed twice with PBS, before being fixed and permeabilized with 3.7% formaldehyde and 0.2% triton x100 for 20 minutes, again at 37°C.

Colonies formed after a seven-day incubation and were enumerated under a fluorescent microscope before being fixed and further stained with trypan blue (Fig. 1A).

To examine the expression profiles of caveolin-1, integrin α7, CTR, emerin and lamin A/C, isolated myofibres were cultured for 48 hrs, before being fixed and immunostained for MyoD and the relevant marker (Figure 3).

Cells were Fc blocked with CD16/32 and stained with FITC – or APC – conjugated CD4, CD8a, or CD8b antibodies (BD Biosciences) before being fixed and permeablized with the Cytofix/Cytoperm kit and stained with PE – conjugated IFNγ (XMG1.2, BD Biosciences).

The cultures were further incubated for 7 10 days before being fixed, stained, and scored for the number of CD59− mutants.

For staining, the cover slips were washed quickly in excess phosphate buffered saline containing 2% bovine serum albumin before being fixed in acetone and air dried.

For tetramethyl rodamine (TMR) labeling, transfected cells were incubated for 15 min with TMR, then the cells were rinsed twice with PBS and incubated in fresh medium for 30 min. The medium was removed, and the cells were washed 3 times with PBS before being fixed with 4% paraformaldehyde and used for imaging.

In addition, we have used a set of central PSFs modelling the Wide Field Camera of the Hubble Telescope in the year 1991, before being fixed, as a realistic (and, at a certain moment, useful) SV restoration problem.

To avoid damage to the fragile borrelial outer membrane, intact bacteria were incubated with antibodies before being fixed onto glass slides and sealed with mounting medium containing the DNA-binding dye DAPI.