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(It lost a further 21% this year, before being fixed at 24,975 to the dollar).
In loliginids they are fertilized as they are ejected and before being fixed in the egg capsule.
They were then washed in distilled water twice before being fixed with 4% paraformaldehyde for 20 min.
Dell's Taiwan website recently listed a 19-inch monitor for the equivalent of about $15 for eight hours before being fixed.
Cells were washed gently with PBS before being fixed with 4% formalin for one hour.
Then the cells were washed again with PBS before being fixed with 70% ethanol at −20C overnight.
The salivary glands were incubated in chromatin wash buffer before being fixed with formaldehyde to remove any unbound Rbf1N-RFP.
Then the cells were washed again with PBS before being fixed with 70% ethanol at −2°C overnight.
After treatment, conceptuses were incubated in KSOM-AA medium for 30 minutes at 37°C before being fixed and processed as described in previous section.
Cells are grown and treated under standard tissue culture conditions before being fixed and printed onto replicate glass slides, effectively decoupling the experimental conditions from the assay technique.
Following incubation cells were washed twice with PBS, before being fixed and permeabilized with 3.7% formaldehyde and 0.2% triton x100 for 20 minutes, again at 37°C.
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CEO of Professional Science Editing for Scientists @ prosciediting.com