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In experiments comparing the appearance of GFP-LC3 puncta to release of cytochrome c, cell were subjected to immunofluorescence staining with cytochrome c antibody before being evaluated under the microscope.
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RTS,S/AS01B had never before been evaluated under field conditions; evaluation of the safety and reactogenicity of RTS,S/AS01B in adults is a crucial requirement before evaluation in children.
Furthermore these processes have to be evaluated under different sizes of π-Clouds according to the before mentioned usage scenarios.
Transgenic lines evaluated under greenhouse condition need to be evaluated under field condition.
These cells were cultivated for 14 days under normoxic condition before the spheres were evaluated under inverse miscopy and counted (more than 30 cells within a sphere was considered to be a full sphere).
Because it will be years before all vehicles can be evaluated under the new system, consumers can look forward to labored explanations of crash-performance ratings by sales personnel for some time to come.
Quality and quantity of RNA was evaluated under UV light before RNA was blotted onto Hybond-N nylon membrane (Amersham) using standard upward blotting technique in 10 × SSC blotting buffer and crosslinked at 120 mJ/cm2 using a UV stratalinker (Stratagene).
All patients were evaluated under stable clinical conditions, before hospital discharge.
All the patients were evaluated under stable clinical conditions, shortly before hospital discharge.
The symptom relief was evaluated under escalating dosage as described before.
5 It is well known that before introducing a new technology into routinely clinical practice, it should be evaluated under highly controlled experimental conditions.
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