Sections were fixed in 2% osmium tetroxide in PB for 1 hr, dehydrated in increasing concentrations of ethanol and propylene oxide before being embedded in EmBed 812 (Electron Microscopy Sciences EMSS)) between two sheets of Aclar plastic (Milner et al., 2011).
SMA wires were programmed before being embedded in the composite beam.
The same beads were functionalized with silane linkers to immobilize the osteoinductive protein BMP-2, and finally received a calcium phosphate coating, before being embedded in the CPC.
Dermal fibroblasts were infected with control adenovirus (second left) or premade adenovirus SDF-1 (second right) before being embedded in collagen lattices.
In some experiments, the dermal fibroblasts were infected with premade adenovirus SDF-1 (Vigene Biosciences, Rockville, MD, USA) before being embedded in collagen lattices.
Before being embedded in Polylite, the sample was covered with an insulating layer to minimize the infiltration into the outer layers.
The spectrum change was not observed when anthracene, before being embedded in KBr, was irradiated with UV in He of 4.0×104 Pa (300 Torr).
Before being embedded in the Polylite resin, samples P-C and P-Au were either coated by carbon (sample P-C) or sputtered by gold (sample P-Au) at the Institute of geochemistry, mineralogy and mineral resources of Charles University in Prague by Prof. Richard Přikryl. .
The specimens were then dehydrated through an ascending series of ethanol and cleared in toluene, before being embedded in paraffin.
All tissues were fixed in 10% neutral buffered formalin for 4 days before being embedded in paraffin wax.
Five-centimeter adjacent segments were also rinsed and fixed in 4 % formaldehyde buffer for 24 h, dehydrated in ethanol, stored at 4°C before being embedded in paraffin.
