Sentence examples for before being developed using from inspiring English sources
The blots were incubated with a rabbit anti-LplA1 antibody (generated against recombinant protein P. falciparum LplA1 by Eurogentec, Belgium) at a dilution of 1∶5000 and the secondary anti-rabbit IgG (H+L), HRP conjugate (Promega) at a dilution of 1∶10,000 before being developed using the Immobilon™ Western Chemiluminescent HRP Substrate (Millipore).
Blots were again washed three times in TBST before being developed using enhanced chemiluminescence (GE Healthcare) and exposure to X-ray film (Fuji).
Membranes were washed in TBS-T (Tween) for 10 min and then placed in horseradish peroxidase secondary antibody for 1 h and washed for 1 h in TBS-T before being developed using a chemiluminescence substance (Thermo Fisher Scientific, Rockford, IL).
These membranes were blocked with 0.1%Tween-20Tween-20 containing 5% non-fat dried milk for 20 min at room temperature, allowed to react with primary antibodies for 2 h, and incubated with a horseradish peroxidase-conjugated goat anti-rabbit or anti-mouse antibody for 2 h before these membranes were developed using SuperSignal ULTRA chemiluminescence substrate (Pierce, Rockford, IL).
The membranes were washed as described before and the blots were developed using ECL Blotting Substrate Thermo Scientificc, Rockford, IL, USA; cat. no. 32209).
Before the steroidal module, ABP has been developed using a Bayesian approach to deter blood doping based on haematological data obtained in whole blood sample.
The membranes were developed using ECL reagents (GE healthcare) before detection using chemiluminescence in a LAS 3000 Fugi imager.
Indeed, fibrate drugs were developed using rodent models to treat hyperlipidemia in humans before the receptor was identified.
Staining was developed using 3,3 diandnobenzidine/H2O2 and sections counterstained with cresyl violet before mounting in DPX (Sigma-Aldrich).
The algorithm was developed using a 25-day monitoring window ending 3 days before hospitalization or death.
