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In all cases, sections were rinsed in TBS several times between and after each incubation and finally transferred onto gelatinized slides before being coverslipped using DPX.
All slides were counter stained by applying 0.5% methyl green for 10 min (blue-green nuclear stain), washed in distilled water, dried in 1-butanol, and transferred to xylene before being coverslipped using Entellan hard mounting medium.
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Slides were then dehydrated by dipping them in 100% ethanol (5×) twice, xylenes (5×), and then allowed to air dry before they were coverslipped using Permaslip® (American MasterTech, Lodi, CA) as the mounting agent.
Slides were coverslipped using Permount.
Dry slides were coverslipped using Vectashield (Vector).
After staining, the slides were coverslipped using Permount (Fisher Scientific, MA).
Finally, sections were coverslipped using DPX (Sigma).
Slides were coverslipped using Faramount mounting medium (Dako).
Slides were dehydrated with ethanol and washed in xylene before being coverslipped and examined using a microscope (Axioplan 2, Carl Zeiss, Germany), and imaging data were acquired by a digital camera (AxioCam MRm, Carl Zeiss, Germany) using Axiovision software (Carl Zeiss, Germany).
Sections were washed in PB, mounted onto slides and left to air dry before being coverslipped and viewed using epifluorescence (Nikon Eclipse 80; Kingston upon Thames, Surrey, United Kingdom).
The sections were finally covered with mounting medium (Life Technologies P369355) containing DAPI before being coverslipped.
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