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The clear supernatant phases were separated in a separating funnel, and the aqueous layer was washed with fresh EtOAc, CHCl3 and hexane (facilitating the concentration of aqueous layers) before being concentrated.
The reaction mixture was stirred for two hours at room temperature before being concentrated.
The resulting solution was stirred at RT for 10.5 h under argon before being concentrated under reduced pressure.
Product was filtered and neutralized before being concentrated to 20 mL using a rotary evaporator (Buchi, New Castle, DE, USA).
The eluted fractions were dialysed against PBS, to remove the free sugar, before being concentrated and quantified.
The isolated Hyd-5 enzyme was subjected to size-exclusion chromatography before being concentrated and entered into crystallization experiments under standard aerobic conditions.
Similar(51)
As schools break up this week in Scotland and with less than a month to go before pupils in England and Wales are set free, more 16- and 17-year-olds than ever before are concentrating on curating their curricula vitae rather than chilling out.
The largest outbreaks of racial violence in Britain before have been concentrated in Afro-Caribbean neighborhoods in the south, like Brixton in London.
Before, I was concentrated on pragmatic issues as prime minister.
What many Cypriots find most frustrating is that their crisis, like those in Ireland and Iceland before them, was concentrated in the banks.
As mentioned before, nomads are concentrated in the western region of the country, where access to health facilities is limited for both nomads and settled communities.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com