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Embryos were collected at 1.5 dpc (1-cell stage), cumulus cells were removed by 0.03% Hyaluronidase, and embryos were allowed to develop in vitro in KSOM+AA (Speciality Media) to various developmental stages before being collected for miRNA profiling.
PC12 cells were treated with NGF for 5 days to allow complete differentiation, and then exposed to VSV-G-enveloped RT43.2GFP vectors for 72 hours before being collected for genomic DNA analysis.
After 11 days the enrichment objects were re-installed into the same cages as before and feathers were allowed to grow for a final 10 days before being collected for analysis.
Cell suspension from ventricular cardiac tissues from day 16 chick embryos were also isolated using a GentleMACSTM dissociator (MACS Miltenyi Biotec, Auburn, CA), preplated, and rotated before being collected for cDNA and proteins.
Cells were seeded at a concentration of 4×10 cells/mL and incubated for 48 h at 37°C, in a humidified atmosphere containing 5% CO2, before being collected for further use.
In this approach, fertilized eggs are injected with a transgene construct and allowed to develop to a pre-determined embryonic stage in utero before being collected for phenotypic analysis.
Similar(54)
During the height of sectarian violence in 2006 and 2007, bodies often remained on the streets for days before being collected.
The advanced state of decomposition of many corpses, some of which were left in the water or sun for days before being collected, hindered efforts by coroners to identify many of the dead.
Runoff is well-mixed and subdivided twice before subsamples are collected for analysis.
Gas released from the ice was dried in a condensation trap (water cooled) and in a P2O5 trap before it was collected for analysis.
Immediately after transfection, cells were grown at 37 °C and 5% CO2 for 3 days before they were collected for genomic modification assays.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com