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The supernatant was centrifuged for at least 30 min at 4 °C and 4500 × g before being clarified through a 0.22 μm membrane (low protein binding Millex-GP polyethersulfone membrane from Millipore, Bedford, U.S.A). to remove the cell debris.
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As it has been clarified before, reproducibility of results is just one of many aspects of reproducibility.
"We were disappointed that the rules hadn't been clarified before," said Ms. Piaia, the executive director of the Chris Hondros Fund, a nonprofit group that supports photojournalism and honors the photographer's memory, in a phone interview.
This office was supposed to handle abuse cases only when they were considered violations of the sacrament of Confession, before the policies were clarified in 2001 and the doctrinal office took on all the abuse cases.
Eurotunnel expects to draw up the rules by the end of the year.Alstom says that it raised concerns months ago that inviting bids for trains with distributed power before these rules were clarified was jumping the gun.
Before fermentation, must was clarified by sedimentation for 24 h at 4°C in the presence of 60 mg l−1 of sulphur dioxide.
The dialysate was clarified as before, and then applied onto a Q Sepharose column (Sigma).
The basic wine is clarified before being placed in the fermentation container.
And even before the terms have been clarified, House and Senate Republicans are attacking the proposal.
But she moved on before the point could be clarified, polling others in the crowd.
But the mixed signals seem unlikely to be clarified before Mr. Annan visits Beirut in a few days.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com