Sentence examples for before being assayed for from inspiring English sources

The zygotes were then left to develop for 20 hours at a constant temperature (22°C) before being assayed for hatching success (see below).

Cells were subsequently cultured for 7 or 14 days before being assayed for levels of transcript reduction, apoptosis and cell cycle profiles relative to unmanipulated cells.

Fourteen clones for each of SS'-derived and FS'-derived subpopulations from each replicate mixture were isolated and conditioned for 24 h before being assayed for growth curve parameters.

Peripheral blood mononuclear cells, platelets and neutrophils were removed from freshly drawn whole blood and pRBCs matured in culture for 24 h before being assayed for rosette and 4-HNE conjugate frequencies as described above.

All cell lines were infected with Chandler/RML prions and cultured for 3 weeks, up to five 1 10 splits, before being assayed for prion propagation as described previously (12).

Single females were then collected after eclosion and allowed to mate with three males for 2 3 days before being assayed for both positional aversion and egg-laying attraction responses to 0.50 mM lobeline.

Moreover, to determine the thermal stability of the enzyme, the r AciHBGase II-(His 6 preparation was prepared in 0.1 M sodium phosphate buffer containing 0.05% (v/v) Triton X-100 at pH 5.5 and kept at the indicated temperature (range 4 85°C) for 15 min before then being assayed for α-glucosidase activity as above.

Microglia were exposed to SiNPs for 24 hr before being assayed.

In transient transfection studies, the medium was then replaced with DMEM with 10% fetal bovine serum and cells were further incubated for 15 h before being assayed.

The medium was then replaced with DMEM with 10% (vol/vol) fetal bovine serum and cells further incubated for 15 h before being assayed.

Transfected cells grown on 96-well plates were washed using a standard buffer (10 mM HEPES, 115 mM NaCl, 1 mM KCl, 1 mM CaCl2, 1 mM MgCl2, 10 mM glucose, pH 7.4), loaded with FLIPR® Membrane Potential Dye (Molecular Devices) according to the manufacturer's instructions, and then incubated at 37 °C for 45 min before being assayed.