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Antibody stained cells where washed using PBS and resuspended in 2% formaldehyde before being analyzed using a FACSCalibur flow cytometer (BD).
The interviews were recorded and transcribed before being analyzed using NVivo 9.
After 4 h incubation, cells were trypsinized and placed on ice before being analyzed using flow cytometry.
The data was checked for errors and missing values before being analyzed using STATA 11.2 (StataCorp, Texas, USA).
The interviews were recorded and transcribed, before being analyzed using an inductive coding technique derived from grounded theory [ 17, 18] to develop a skills taxonomy.
Based on pretesting, the extracted DNA were diluted (1 100) with molecular-grade, nuclease-free water before being analyzed using qPCR to minimize inhibitory effects of extraneous matter in the samples.
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Milk samples (20 mL of milk) were thawed and centrifuged at 3500 g for 10 min at 10 °C before they were analyzed using an ELISA kit for M1.
If not, data were log transformed to achieve normality of distribution before they were analyzed using factorial ANOVA.
The CA9 expression of primary breast tumors before NAC was analyzed using CNB specimens.
Propidium iodide (PI) (1.4 μg/ml) was added 5 min before cells were analyzed using an LSRII flow cytometer (BD Biosciences, San Jose, CA, USA).
The mixtures were boiled in a water bath for 15 min and left at room temperature for 10 min before they were analyzed using a UV/VIS Spectra Lambda 35 (PerkinElmer).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com