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Following two PBS washes, cells were fixed in 0.1% formaldehyde overnight before being analysed using a FACScalibur flow cytometer (Beckton Dickinson).
The cells were then incubated with Alexa Fluor 488 donkey anti-goat secondary antibody (1/800 dilution) for 30 min at room temperature before being analysed using a flow cytometer.
Data were obtained in Excel and edited in SPSS (V.19·0. Armonk, New York, USA: IBM) before being analysed using the freeware statistical program R V.2·5·2.
In vivo and HR-MAS H MR spectra were automatically phased and referenced to the creatine peak at 3.03 p.p.m. before being analysed using the TARQUIN algorithm for in vivo MRS as described by workers Wilson et al (2010).
The cotton swab was then centrifuged (Hettich Centrifuge EBA 20, Andreas Hettich Group, Ltd., Tuttlingen, Germany) at 2400 x g at room temperature for 2 minutes and the saliva extracted was stored at −20°C before being analysed using a commercially available cortisol ELISA kit (Cortisol ELISA, IBL International, Hamburg, Germany) validated for pig saliva [ 18].
The data were checked for errors and missing values before being analysed using STATA 11.2 (StataCorp, Texas, USA).
Similar(54)
Differences in characteristics between patients with IC and with CLI before revascularization were analysed using Chi-squared test and Mann-Whitney U-test.
Five paired samples from oesophageal cancer patients before and after chemotherapy were analysed using the same methodology.
The differences in biomarker levels before and after intubation were analysed using repeated measures analysis of variance and a paired t test with correction for multiple comparisons.
The three feedback sessions both before and after the intervention were analysed using the coding list described above.
Changes in plasma lyso-Gb3, eGFR, urine ACR, LVM, LVMI, IVS, LPW and MSSI before and after treatment were analysed using Student's paired t test.
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