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Many of the artists Dia has supported, including Andy Warhol, Joseph Beuys, Donald Judd, Dan Flavin and Richard Serra, have had individual shows at Dia's Manhattan headquarters, but the center's permanent collection has never before been viewed in one place.
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The humorous spot, which carried the theme "Open your world," made its way onto TV in about 30 countries three months later, but not before being viewed frequently online.
They were gold coated under reduced pressure with a sputter coater before being viewed under the SEM at 20 kV.
The sections were stained with lead citrate and uranyl acetate before being viewed in a Phillips 100CS electron microscope.
They were counterstained with bisbenzimide (2.5 μg/100 mL, Hoechst 33258; Sigma) and mounted using 0.1 M phosphate buffer (PB) before being viewed on an upright epifluorescence microscope (Leica).
After being embedded in epon, ultrathin sections were mounted on metal grids and stained with uranyl acetate before being viewed in a transmission electron microscope JEM-12300; JEOL, Tokyo, Japan).
The stained slides were rinsed thoroughly with running tap water followed by distilled water and dried before being viewed under the microscope.
After dehydration with a graded acetone series, specimens were dried with liquid CO2 and coated with a thin layer of gold before being viewed under an S-3000 N scanning electron microscope.
For immune EM, cryo-sections were post labeled using monoclonal anti-HA (1 60 in blocking buffer containing 1% BSA, 0.5% CWFSG and 0.01% BSA-C in PBS) and rabbit anti mouse antibodies (1: 250 in blocking buffer) and a protein A-gold 10 nm conjugate (in 1% BSA in PBS) before being viewed in a Jeol 1010 electron microscope (Jeol, Tokyo, Japan) operated at 80 kV.
Before then, China had been viewed simply as a promising commercial market.
But it is certain, people around the region said, that it will be a long time before Iraq is viewed broadly as free and democratic.
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