Highly c-axis-oriented BTO thin films reported before are grown on either a single-crystalline oxide substrate or with a preferentially oriented thick (>100 nm) conductive or dielectric intermediate buffer layer[13, 15].
In Figure 5, cells were streaked once to solid minimal medium lacking tryptophan before being grown for ChIP.
Transformants were screened by FACS analysis before being grown in the absence of tetracycline to select cell lines with optimal control of expression.
Unless noted otherwise, all accessions were collected in natural populations and self-fertilized two generations before being grown for DNA extraction.
The seeds were chilled at 4°C in the dark for 5 days before being grown in soil medium (Bio-mix TTing Substratum, Moerdijk, Nederland).
Surface sterilized seeds were stratified for 4 days in the dark at 4°C before being grown under cool-white fluorescent tubes (70 100 μmol m−2 s−1) in LD cycles at constant 22°C.
Briefly, leaf discs (0.5 cm × 0.5 cm) were dipped in Agrobacterium overnight culture (OD600 = 0.6), regenerated under kanamycin selection (30 μg/ml) and rooted plantlets hardened off before being grown in a greenhouse under conditions described above.
For clonogenic assays, cells were infected with shRNAs at low MOI in six-well plates (three per shRNA) and selected with puromycin for 3 days before being grown in drug-free media for 10 14 days.
The discrepancy may be explained by differences in the growth conditions since in the above study [ 14], seeds were germinated in light for two to six hours before being grown in the dark for three days in the presence of PAC, whereas in the study analyzed here, seeds were stratified and germinated in the presence of light and PAC or ABA [ 60].
Monocyte-derived macrophages were obtained by adherence of fresh monocytes, in serum-free medium, to glass coverslips for 1 h before being grown on in RPMI supplemented with 10% fetal bovine serum (FBS), Glutamax, pyruvate, penicillin/streptomycin, non-essential amino acids and 50 ng/ml M-CSF for 6 or 7 days at which point cells were spread and strongly adherent.
