Sentence examples for before assay by using from inspiring English sources

All plasma samples were kept at −80°C before assay by using a commercially available corticosterone ELISA kit (Cayman Chemical, USA).

For functional studies, cells were transfected by electroporation using the AMAXA system (Amaxa GmbH, Cologne, Germany), plated on 96 well plates and incubated 1 2 days before assay.

(A) The cytotoxicity of 924-based ITs was assayed by using persistently-infected H9/NL4-3 cells.

Cells were pelleted and resuspended in 55 mM HCl containing 5 mg/ml Proteinase K, incubated at 37°C for 30 min, washed once with 200mM Tris pH 7.5, 211 mM NaCl, 78mM MgCl2, then resuspended in the same buffer with 1mg/ml of propidium iodide before assaying by flow cytometry using a FACScan cytometer (Becton Dickinson) and CellQuest Pro software.

The observation that stabilized blood samples can be stored at 4 °C for up to 96 h before analysis by using the single-platform assay is particularly important for the application of the assay in multicenter clinical trials, which require all analyses to be performed at a central laboratory.

Myocardial caspase-3 activity was determined as described before [ 21] by using a caspase colorimetric assay kit (Chemicon, Temecula, CA, USA) according to manufacturer's protocol.

Cancer cells cytotoxic assay were done by using MTS.

ChIP assays were done by using protein A-coated 96-well polypropylene microplates as described before [ 27].

Cell viability was evaluated before and after HCl administration by using a cytotoxicity assay (Roche GmbH, Mannheim, Germany).

HUVEC was labeled GFP by using lentivirus before this assay.

Then, post-treatment values were obtained by using the alamarBlue assay as described before.