Then, samples were put in deionized water for the next 14 h to achieve fully swollen state before experimental analysis.
Before experimental analysis, the two-month-old seedlings of D. officinale were trimmed to provide uniformly sized materials, each consisting of a stem supporting three to five leaves and two to three roots of 20 mm in length.
Therefore, predicting good candidate genes before experimental analysis will save time and effort.
Furthermore, the methods that we will develop on 7 - 11 phage can be directly tested on some of the bacteriophages that express their own σ factor, and for which the complete experimental analysis was done before.
Experimental analysis applied with one-way ANOVA.
Further experimental analysis of the post-transcriptional and pre-translational effect of altering these sequences is necessary before any biological significance can be attributed to these observations.
WEB performed experimental analysis.
Animals were allowed to acclimate to the laboratory for 7 days before any experimental manipulation.
It should be noted that MDA-MB-231 is already aneuploid before any experimental handling.
All cells were incubated for 24 hours at 37°C before starting any experimental procedure.
